Regulatory

Part:BBa_K368024:Design

Designed by: Jara Radeck   Group: iGEM10_LMU-Munich   (2010-10-24)


tet-on Promoter (prev TRE) + attB (bacterial attachment site)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

integrate the eGFP sequence where attB site is, so that eGFp will be translated after induction with docycycline


Source

from a plasmid

References